By Alton Parrish.
Image: Carly Sanker/MIT
Sallie Chisholm, the Lee and Geraldine Martin Professor of Environmental Studies in CEE and in MIT’s Department of Biology; former CEE postdoc Nadav Kashtan; and co-authors published a paper on this work in the April 25 issue of Science.
The researchers estimate that the subpopulations diverged at least a few million years ago. The backbone is an older, more slowly evolving component of the genome, while the flexible genes reside in areas of the genome where gene exchange is relatively frequent, facilitating more rapid evolution.
The study also revealed that the relative abundance of the backbone subpopulations changes with the seasons at the study site, near Bermuda, adding strength to the argument that each subpopulation is finely tuned for optimal growth under different conditions.
“The sheer enormity of diversity that must be in the octillion Prochlorococcus cells living in the seas is daunting to consider,” Chisholm says. “It creates a robust and stable population in the face of environmental instability.”
Scientists on an oceanographic research ship lower a rosette of 24 bottles into the ocean to capture samples of wild microbes in their natural habitat.
Photo: Paul Berube/MIT
“The interesting question is, ‘Why does such a diverse set of subpopulations exist?’” Kashtan says. “The huge population size of Prochlorococcus suggests that this remarkable diversity and the way it is organized is not random, but is a masterpiece product of natural selection.”
Chisholm and Kashtan say the evolutionary and ecological distinction among the subpopulations is probably common in other wild, free-living (not attached to particles or other organisms) bacteria species with large populations and highly mixed habitats.
“This is perhaps the most sophisticated and thorough study yet to be published on the fine-scale genetic diversification of an environmental microbial species, and it correctly, I think, predicts that amazingly diverse populations may be maintained over astonishingly long times,” says Ford Doolittle, a member of the biochemistry and molecular biology faculty at Dalhousie University who was not involved in the research. “If microbiologists persist in believing in ‘species,’ they will likely have to drastically revise upward their estimates of how many such things there are. What we will probably be arguing about for a long time is what processes or forces other than selection might be responsible for such stable diversity, and, unless we find such processes, how something so seemingly well mixed as the ocean can offer up so many different tiny selective regimes.”
Scientists on an oceanographic research ship lower a rosette of 24 bottles into the ocean to capture samples of wild microbes in their natural habitat.
Photo: Paul Berube/MIT
“This study may be setting a record for progress made in microbiology by analyzing just three drops of seawater,” says Ramunas Stepanauskas of the Bigelow Laboratory for Ocean Sciences in Boothbay, Maine, who worked on the genomic analysis.
Other co-authors are Sara Roggensack, Sébastien Rodrigue, Jessie Thompson, Steven Biller, Allison Coe, Huiming Ding, Roman Stocker, and Michael Follows of MIT; Pekka Marttinen of the Helsinki Institute for Information Technology; and Rex Malmstrom of the Department of Energy’s Joint Genome Institute.
The work was supported by the National Science Foundation (NSF) Division of Environmental Biology, the NSF Biological Oceanography Section, the NSF Center for Microbial Oceanography Research and Education (C-MORE), the U.S. Department of Energy’s Genomics Science Program, and the Gordon and Betty Moore Foundation Marine Microbiology Initiative.
